Expedeon InstantBlue蛋白電泳染色劑

Expedeon InstantBlue蛋白電泳染色劑

Expedeon 公司是英國一家專注于生物偶聯(lián)技術(shù)（bioconjugation）的企業(yè)。生物偶聯(lián)即將兩種分子結(jié)合在一起而形成一個雜合的偶聯(lián)物（hybrid conjugate），這些偶聯(lián)結(jié)合物（如標(biāo)記抗體）在世界各大醫(yī)院和科研機構(gòu)被廣泛使用。Expedeon 公司開發(fā)生產(chǎn)“一步法”抗體標(biāo)記試劑盒-Lightning-Link™系列產(chǎn)品極大程度的簡化了結(jié)合標(biāo)記流程，是目前世界上操作較簡便的抗體標(biāo)記試劑盒。

  上海易匯生物科技有限公司擁有強大的采購系統(tǒng)。只要您想得到。沒有公司采購辦不到的。
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  上海易匯生物科技有限公司直接與廠家合作。保證貨源正品。購買無后顧之憂。
  上海易匯生物科技有限公司保證100%。保證100%產(chǎn)品都是正品。
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張經(jīng)理：Q821536999

上海易匯生物科技有限公司代理

     在進行多因子檢測時，我們往往因不能找到多種合適的標(biāo)記一抗，而不得不選用標(biāo)記二抗，因此很難避免可能發(fā)生的交叉反應(yīng)及繁瑣的洗滌步驟。Lightening-LinK™抗體標(biāo)記試劑盒系列，提供多種熒光蛋白、酶及熒光素標(biāo)記等產(chǎn)品，試驗操作人員可直接應(yīng)用于標(biāo)記一抗，從而避免了因使用二抗帶來的交叉反應(yīng)及非特異性結(jié)合等問題。該系列試劑盒具有以下顯著特點

特點 優(yōu)勢

● 操作簡單：一步法標(biāo)記

● 快速: 手工操作時間僅30秒 ● 目前世界上操作較簡便的抗體標(biāo)記試劑盒

● 無需脫鹽、透析等步驟 ● 抗體回收率達100%

● 可提供50種標(biāo)記物 ● 可用于WB,IHC，ELISA,免疫熒光、流式細胞術(shù)以及熒光共振能量轉(zhuǎn)移檢測等

● 經(jīng)濟實惠，規(guī)格多樣 ● 可標(biāo)記ug-mg 級抗體量

Lighting-Link™系列與其他品牌的抗體標(biāo)記試劑盒比較

 廠 家 Supplier I1 Supplier I2 Supplier I3 Supplier S1 Supplier S2 Supplier D Lighting-Link

 手工操作時間 2 分鐘 20 分鐘 30 分鐘 30 分鐘 30 分鐘 10 分鐘 30 分鐘

 是否過柱 不需要 需要 需要 需要 需要 需要 不需要

 試劑盒組分 6 組分 7 組分 5 組分 9 組分 612 組分 6 組分 3 組分

 操作步驟 5 步 11 步 16 步 27 步 31 步 8 步 3 步

 抗體標(biāo)記時間 10 分鐘   2.5 小時 2 小時 1.5 小時 4 小時 1 小時 2 小時

 可標(biāo)記抗體量 1-20ug 10-20ug 20-100ug 25ug-5mg 100ug 50-200ug 100ug-5mg

 連接化學(xué)健 非共價結(jié)合* 共價結(jié)合 共價結(jié)合 共價結(jié)合 共價結(jié)合 共價結(jié)合 共價結(jié)合

 抗體亞型 僅IgG 僅IgG 所有 僅IgG 僅IgG 僅IgG 所有

*非共價連接的抗體標(biāo)記物只能穩(wěn)定30分鐘

試劑盒組成

● 1或3(以規(guī)格而定)玻管Lightening-Link™ Mix 混合物。 

● 1管LL-X（X為標(biāo)記物，如PE或HRP等）修飾試劑。 

● 1管LL-X萃滅試劑。

操作步驟

1）在每10ul抗體樣品中加入1ul的LL-Modifier修飾試劑，輕輕混勻

2）打開Ligtening-Link混合物管蓋，吸取步驟1已混好的抗體樣品至管內(nèi)凍干混合物中，用吸液器輕輕吸打1-2次,使粉末*溶解

3）蓋上玻璃瓶蓋，室溫(20-25°C)避光孵育3小時,或過夜

4）孵育3小時后，按每10ul抗體的量加入1ul LL-淬滅試劑，靜置30分鐘后即可使用

 *不同的標(biāo)記物，操作步驟略有不同。（操作步驟供參考，以英文原版說明書為準

標(biāo)記結(jié)合物的保存

對于任何新結(jié)合物，推薦保持于4°C條件下。如需長期保存，可添加保護劑

緩沖液及添加劑兼容性：

A. 與無胺基緩沖液兼容，包括MES,MOPS,HEPES（濃度10-50mM, pH范圍6.5-8.5）,LL-Modifier修飾劑提供結(jié)合反應(yīng)的條件

B. 常見的鹽（如氯化鈉）、螯合劑（EDTA）、糖對偶聯(lián)反應(yīng)無影響

C. 疊氮鈉（0.02%-0.1%）對結(jié)合反應(yīng)有輕微影響，疊氮鈉不可用于HRP標(biāo)記，其為后者的抑制劑

D. 甘油(up to 50%)對結(jié)合效率無影響

E. 若無胺基的緩沖液濃度相對較高，pH值在6.6-8.5范圍外，則需要加入更多的LL-midifier修飾劑（/10ul抗體），以此來調(diào)整pH值。的pH范圍為7.3-7.6左右

F. 避免使用含親核劑的緩沖液，避免還原劑如DTT和2-巰基乙醇，乙醇胺等

G. 濃度達到或稍低于20mM的Tris對結(jié)合效率有輕微的影響

Lightening-Link™ 抗體/蛋白標(biāo)記試劑盒

● 酶標(biāo)記試劑盒

Lightning-Link® Alkaline Phosphatase (AP)

Lightning-Link® Glucose Oxidase

Lightning-Link® Horseradish Peroxidase (HRP)

● 及鏈霉親和素標(biāo)記試劑盒

Lightning-Link® Avidin

Lightning-Link® Biotin

Lightning-Link® Streptavidin

Expedeon 701-0000 Lightning-Link® HRP Labeling Kit Ambient temperature ￥4,019.10

Expedeon 701-0002 Lightning-Link® HRP Labeling Kit Ambient temperature ￥6,895.71

Expedeon VGE-1012 Gel Wrap Gasket 1mm for 14.5cm plates Room Temperature ￥196.98

Expedeon VGE-1016 Gel Wrap Gasket 1mm for 16.5cm plates Room Temperature ￥196.98

Expedeon VGE-1017 Gel Wrap Gasket 1mm for 17cm plates Room Temperature ￥196.98

Expedeon VGE-1019 Gel Wrap Gasket 1mm for 19cm plates Room Temperature ￥253.26

Expedeon VGE-1020 Gel Wrap Gasket 1mm for 22cm plates Room Temperature ￥253.26

Expedeon VGE1020-177 Gel Wrap Gasket 1mm for DGGE Room Temperature ￥295.47

Expedeon VGE-1025 Gel Wrap Gasket 1mm for 28cm plates Room Temperature ￥295.47

Expedeon VGE-1040 Gel Wrap Gasket 1mm for 38.7cm plates Room Temperature ￥351.75

Expedeon VGE-10XX Gel Wrap Gasket 1mm per foot Room Temperature ￥126.63

Expedeon VGE-1512 Gel Wrap Gasket 1.5mm for 14.5cm plates Room Temperature ￥196.98

Expedeon VGE-1516 Gel Wrap Gasket 1.5 mm for 16.5cm plates Room Temperature ￥196.98

Expedeon VGE-1517 Gel Wrap Gasket 1.5mm for 17cm plates Room Temperature ￥196.98

Expedeon VGE-1519 Gel Wrap Gasket 1.5 mm for 19cm plates Room Temperature ￥253.26

Expedeon VGE-1520 Gel Wrap Gasket 1.5mm for 22cm plates Room Temperature ￥253.26

Expedeon VGE1520-177 Gel Wrap Gasket 1.5mm for DGGE Standard conditions - no special allowances needed ￥267.33

Expedeon VGE-1525 Gel Wrap Gasket 1.5mm for 28cm plates Room Temperature ￥295.47

Expedeon VGE-1540 Gel Wrap Gasket 1.5mm for 38.7cm plates Room Temperature ￥351.75

WMHE-600 Flexicast Midi Horizontal, 16cm(w) x 16cm(l) Kit Standard conditions - no special allowances needed 訂購詢價

Expedeon WPS-100 Wedge Plate Separators Room Temperature ￥140.70

Expedeon NXB86425 RunBlue Tris-Glycine Blot Buffer - 4L (10x Concentrate) Room Temperature ￥0.00

Expedeon NXB86500 RunBlue Tris-Glycine Blot Buffer - 500 ml (10x Concentrate) Room Temperature ￥0.00

Expedeon NXB87500 InstantBlot Transfer Buffer 500ml 10X Room Temperature ￥0.00

Expedeon NXG00812 RunBlue SDS Gel 8% 12 well - 10x10 4°C ￥0.00

Expedeon NXG00827 RunBlue SDS Gel 8% 17 well - 10x10 4°C ￥0.00

Expedeon NXG01012 RunBlue SDS Gel 10% 12 well - 10x10 4°C ￥0.00

Expedeon NXG01027 RunBlue SDS Gel 10% 17 well - 10x10 4°C ￥0.00

Expedeon NXG01212 RunBlue SDS Gel 12% 12 well - 10x10 4°C ￥0.00

Expedeon NXG01227 RunBlue SDS Gel 12% 17 well - 10x10 4°C ￥0.00

Expedeon NXG01612 RunBlue SDS Gel 16% 12 well - 10x10 4°C ￥0.00

Expedeon NXG01627 RunBlue SDS Gel 16% 17 well - 10x10 4°C ￥0.00

Expedeon NXG40812 RunBlue SDS Gel 4-8% 12 well - 10x10 4°C ￥0.00

Expedeon NXG40827 RunBlue SDS Gel 4-8% 17 well - 10x10 4°C ￥0.00

Expedeon NXG41212 RunBlue SDS Gel 4-12% 12 well - 10x10 4°C ￥0.00

Expedeon NXG41227 RunBlue SDS Gel 4-12% 17 well - 10x10 4°C ￥0.00

Expedeon NXG42002 RunBlue SDS Gel 4-20% 2 well - 10x10 4°C ￥0.00

Expedeon NXG42012 RunBlue SDS Gel 4-20% 12 well - 10x10 4°C ￥0.00

Expedeon NXG42027 RunBlue SDS Gel 4-20% 17 well - 10x10 4°C ￥0.00

Expedeon QNC-700 COOLED QUADRA MINI-SYSTEM, CE, Room Temperature ￥11,818.80

Expedeon QNX-700 QUADRA MINI-SYSTEM, CE Room Temperature ￥9,412.83

Expedeon QT-100-2 QUICKTRAP VACUUM PROTECTION SYS, 2 Liter Room Temperature ￥8,990.73

Expedeon QT-100-5 QUICKTRAP VACUUM PROTECTION SYS, 5 Liter Room Temperature ￥10,271.10

Expedeon SG-125 C.B.S. LITE VERTICAL SYSTEM Room Temperature ￥7,682.22

Expedeon SG-125-02 C.B.S. LITE VERTICAL SYSTEM,CE Room Temperature ￥8,864.10

Expedeon SG-200 C.B.S. LITE VERTICAL SYSTEM Room Temperature ￥7,949.55

Expedeon SG-200-02 C.B.S. LITE VERTICAL SYSTEM,CE Room Temperature ￥9,103.29

Expedeon SG20-0417 Rectangular tooth comb 0.4mm x 17 well Standard conditions - no special allowances needed ￥731.64

Expedeon SG20-0420 Rectangular tooth comb 0.4mm x 20 well Room Temperature ￥731.64

Expedeon SG20-0428 Rectangular tooth comb 0.4mm x 28 well Standard conditions - no special allowances needed ￥745.71

Expedeon SG20-0717 Rectangular tooth comb 0.75mm x 17 well Room Temperature ￥759.78

Expedeon SG20-0720 Rectangular tooth comb 0.75mm x 20 well Room Temperature ￥759.78

Expedeon SG20-0728 Rectangular tooth comb 0.75mm x 28 well Room Temperature ￥773.85

Expedeon SG20-1017 Rectangular tooth comb 1mm x 17 well Room Temperature ￥759.78

Expedeon SG20-1020 Rectangular tooth comb 1mm x 20 well Room Temperature ￥759.78

Expedeon SG20-1028 Rectangular tooth comb 1mm x 28 well Room Temperature ￥801.99

Expedeon SG20-1517 Rectangular tooth comb 1.5mm x 17 well Room Temperature ￥759.78

Expedeon SG20-1520 Rectangular tooth comb 1.5mm x 20 well Room Temperature ￥759.78

Expedeon SGC14-2027 Standard Comb 2mm x 27 well Standard conditions - no special allowances needed ￥745.71

Expedeon SGC20-1016 Standard Comb 1.0mm x 16 well Room Temperature ￥745.71

Expedeon SGC20-1020 Standard Comb 1mm x 20 well Standard conditions - no special allowances needed ￥745.71

Expedeon SGC20-1040 Standard Comb 1mm x 40 well Room Temperature ￥998.97

Expedeon SGC20-1520 Standard Comb 1.5mm x 20 well Standard conditions - no special allowances needed ￥745.71

Expedeon SGC20-1540 Standard Comb 1.5mm x 40 well Room Temperature ￥998.97

Expedeon SGC20-2020 Standard Comb 2mm x 20 well Standard conditions - no special allowances needed ￥745.71

Expedeon SGC20-2040 Standard Comb 2mm x 40 well Room Temperature ￥998.97

Expedeon SGC26-1026 Standard Comb 1mm x 26 well Standard conditions - no special allowances needed ￥801.99

Expedeon SGC26-1051 Standard Comb 1mm x 51 well Room Temperature ￥1,097.46

Expedeon SGC26-1526 Standard Comb 1.5mm x 26 well Standard conditions - no special allowances needed ￥801.99

Expedeon SGC26-1551 Standard Comb 1.5mm x 51 well Room Temperature ￥1,097.46

Expedeon SGC26-2026 Standard Comb 2.0mm x 26 well Standard conditions - no special allowances needed ￥801.99

Expedeon SGC26-2051 Standard Comb 2.0mm x 51 well Room Temperature ￥1,097.46

Expedeon SGE50-1020 Gel Wrap Gasket, 1mm x 22cm Room Temperature ￥450.24

Expedeon SGE50-1030 Gel Wrap Gasket, 1mm x 32cm Room Temperature ￥450.24

Expedeon SGE50-1520 Gel Wrap Gasket, 1.5mm x 22cm Room Temperature ￥450.24

Expedeon SGE50-1530 Gel Wrap Gasket, 1.5mm x 32cm Room Temperature ￥450.24

Expedeon SGP20-020 Glass Plate Set, 20cm(w) x 22cm(h) Room Temperature ￥970.83

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